An anaerobic chamber is an essential piece of equipment in microbiology research. When research processes involve bacteria or microorganisms that are anaerobic, this equipment must be used to protect them from the ambient air. The interior volume of the anaerobic chamber contains no oxygen, allows these types of bacteria to be cultivated away from the ambient air.
Anaerobic Chamber: Characteristics
An anaerobic chamber allows the incubation of anaerobes. By this, we mean all bacteria that are able to live and develop without oxygen and are behind three families of infection: intoxications, gas gangrene and mixed infections. In anaerobiosis or in the absence of oxygen, their strains grow under optimal conditions. The anaerobic chamber can also be referred to by other names: Freiter’s chamber or incubator, enclosure or anaerobic station.
How does the anaerobic enclosure create anaerobiosis? A purification device using gas mixtures is integrated into this equipment. These gases, often H2, N2 or others, rid the internal environment of oxygen. The anaerobic chamber also has temperature regulators allowing the control of the temperature according to the conditions required by the incubation. And in order to maintain anaerobic conditions, it is equipped with components that facilitate the monitoring of each Petri dish, in particular to see how the microbial culture evolves.
How to Grow Bacteria in an Anaerobic Chamber?
Microbial culture in anaerobic conditions generally goes through several phases. During microbiological research such as phenotypic studies, bacteria are collected and transported, for example, through blood culture. This bacteriological technique consists of inoculating two vials, one of which contains oxygen and one of which does not. They are then cultured in an anaerobic chamber.
They are usually incubated in blood agar for a few hours, sometimes for a few days. The temperature of the medium should be around 37°C. Thanks to the anaerobic chamber, the operators can examine each sample and handle them with sleeves from the outside, which prevents the anaerobiosis from breaking. The device is used to grow different kinds of strains: micro-aerophilic organisms, hypoxic tissues or cells, etc. The anaerobic chamber is also used to grow a wide range of strains. Its great advantage is that it optimizes sample protection by minimizing the risk of sample contamination.
Example with Jacomex Manufacturer
Jacomex is a world-renowned expert on this subject. As a company specialized in the design and manufacture of containment solutions, it offers ranges of high-quality anaerobic chambers to the scientific community.
GP(Campus) Glove Box
It offers this range of purified and standard glove boxes to medical, microbiological, research laboratories, etc. Equipped with a compact purification unit (<1 ppm O2/H2O), they are distinguished by their high performance. They can be used for all applications requiring a controlled atmosphere, including anaerobic experiments.
GP(Concept) Glove Box
The GP (Concept) range includes purified (<1 ppm O2/H2O) and versatile glove boxes designed to protect applications and operators. It offers an increased level of safety as an anaerobic chamber. There are standard glove boxes in this range, but Jacomex also offers customization options. Depending on the customer’s specifications, the manufacturer can customize the dimensions, add work faces and additional accessories.